Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Target-dependent nickase activities of the CRISPR-Cas nucleases Cpf1 and Cas9. Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis. The new frontier of genome engineering with CRISPR-Cas9. ![]() Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases. Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. ![]() Nat Biotechnol 36, 265–271.Ĭhen, J.S., Dagdas, Y.S., Kleinstiver, B.P., Welch, M.M., Sousa, A.A., Harrington, L.B., Sternberg, S.H., Joung, J.K., Yildiz, A., and Doudna, J.A. A highly specific SpCas9 variant is identified by in vivo screening in yeast. Casini, A., Olivieri, M., Petris, G., Montagna, C., Reginato, G., Maule, G., Lorenzin, F., Prandi, D., Romanel, A., Demichelis, F., et al.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |